Fig. 1.

Recombinant FN domains synthesized. a Domain structure of FN comprising the type I, II and III repeats. The affinity of certain domains for specific molecules e.g. fibrin, heparin and collagen and the location of splice variants ED-B, ED-A, V and C regions are shown. b Regions covered by the recombinant proteins that were synthesized with the corresponding amino acid residues and the molecular weight of each protein. c Coomassie-stained gel showing 10 μg of each recombinant protein analyzed by SDS-PAGE (12% gel) under reducing conditions. Lanes: 1 = FN III 12–14, 2 = FN III 13–14, 3 = FN III 12, 4 = FN III 13, 5 = FN III 14, 6 = V region.