PF-QNM images at the lipid phase boundary showing the topography (nm), stiffness (MPa), and deformation (nm) at 200 pN peak loading force. The false color scale (from top to bottom) is 6 nm, 60 MPa, and 4 nm. Cross section of the trace (blue line) and retrace (red line) along the white dashed line for topography, stiffness, and deformation (average of three consecutive lines).