Figure 2.

Fibrin fiber mechanical properties. (A–C) Optical images of γQ398N/Q399N/K406R fiber extensibility measurement. The AFM tip is indicated by a green asterisk; the fibrin fiber, attached to 7 μm wide ridges (white bands), extends across a 13 μm wide grove (dark band). (C) Fiber ruptures at a strain of 350%. (D) Top view schematic of the experimental setup. The initial, unstretched fiber, with length L_initial, is shown in gray; the stretched fiber, with length, L', is shown in black, and the AFM tip is shown as a green dot. The AFM tip travels a distance s, using the Pythagorean Theorem, the fiber is stretched to L' = (s² + L_initial²)^1/2. s is obtained from the nanoManipulator AFM data file, L_initial is determined from the optical image.