Table 1.

Comparison of V. cholerae O1 counts in Mathbaria pond water (MW) microcosms with and without chitin^a,b.

Day	Type of microcosm	Plate count (cfu/ml)		AgglutinateC	DFA	Multiplex-PCR
		TCBS	LBA		Cells/ml	O1 wbe	ctxA
1	V. cholerae O1 in microcosm MW–CC	2.0 × 106	3.8 × 107	+	3.8 × 108	+	+
	V. cholerae O1 in microcosm MW	2.0 × 106	3.8 × 107	+	3.8 × 108	+	+
7	V. cholerae O1 in microcosm MW–CC	2.1 × 103	1.1 × 104	+	+d	+	+
	V. cholerae O1 in microcosm MW	2.8 × 103	3.8 × 104	+	1.8 × 108	+	+
14	V. cholerae O1 in microcosm MW–CC	1.4 × 103	1.9 × 104	+	BFd	+	+
	V. cholerae O1 in microcosm MW	1.9 × 103	1.7 × 104	+	1.8 × 108	+	+
21	V. cholerae O1 in microcosm MW–CC	2.0 × 103	2.4 × 104	+	BFd	+	+
	V. cholerae O1 in microcosm MW	1.6 × 103	2.8 × 104	+	BF	+	+
28	V. cholerae O1 in microcosm MW–CC	1.9 × 103	1.3 × 104	+	BFd	+	+
	V. cholerae O1 in microcosm MW	1.1 × 103	4.8 × 104	+	BF	+	+
35	V. cholerae O1 in microcosm MW–CC	1.6 × 102	1.1 × 103	+	BFd	+	+
	V. cholerae O1 in microcosm MW	1.7 × 101	2.4 × 102	+	BF	+	+
42	V. cholerae O1 in microcosm MW–CC	1.6 × 101	2.4 × 103	+	BFd	+	+
	V. cholerae O1 in microcosm MW	<10	1.0 × 102	+	BF	+	+
49	V. cholerae O1 in microcosm MW–CC	7.7 × 101	2.4 × 102	+	BFd	+	+
	V. cholerae O1 in microcosm MW	<10	6.8 × 101	+	BF	+	+
56	V. cholerae O1 in microcosm MW–CC	6.7 × 101	1.4 × 102	+	BFd	+	+
	V. cholerae O1 in microcosm MW	<10	<10	NG	BF	ND	ND
63	V. cholerae O1 in microcosm MW–CC	6.5 × 101	1.2 × 102	+	BFd	+	+
	V. cholerae O1 in microcosm MW	<10	<10	NG	BF	ND	ND
70	V. cholerae O1 in microcosm MW–CC	6.4 × 101	1.0 × 102	+	BFd	+	+
	V. cholerae O1 in microcosm MW	<10	<10	NG	BF	ND	ND
76	V. cholerae O1 in microcosm MW–CC	4.0 × 101	5.0 × 101	+	BFd	+	+
	V. cholerae O1 in microcosm MW	ND	ND	NG	ND	ND	ND
92	V. cholerae O1 in microcosm MW–CC	<10	1.1 × 101	+	ND	+	+
	V. cholerae O1 in microcosm MW	ND	ND	NG	ND	ND	ND
99	V. cholerae O1 in microcosm MW–CC	<10	2.5 × 101	+	ND	+	+
	V. cholerae O1 in microcosm MW	ND	ND	NG	ND	ND	ND
113	V. cholerae O1 in microcosm MW–CC	ND	2.1 × 101	+	ND	+	+
	V. cholerae O1 in microcosm MW	ND	ND	NG	ND	ND	ND
132	V. cholerae O1 in microcosm MW–CC	ND	2.0 × 101	+	ND	+	+
	V. cholerae O1 in microcosm MW	ND	ND	NG	ND	ND	ND
146	V. cholerae O1 in microcosm MW–CC	ND	2.1 × 101	+	ND	+	+
	V. cholerae O1 in microcosm MW	ND	ND	NG	ND	ND	ND
160	V. cholerae O1 in microcosm MW–CC	ND	2.1 × 101	+	ND	+	+
	V. cholerae O1 in microcosm MW	ND	ND	NG	ND	ND	ND
174	V. cholerae O1 in microcosm MW–CC	ND	1.0 × 101	+	ND	+	+
	V. cholerae O1 in microcosm MW	ND	ND	NG	ND	ND	ND
189	V. cholerae O1 in microcosm MW–CC	ND	<10	NG	BFd	+e	+e
	V. cholerae O1 in microcosm MW	ND	ND	NG	ND	ND	ND

^aMicrocosm was constructed using Mathbaria pond water, a known reservoir of V. cholerae (Alam et al., 2006a).

^bMicrocosm was constructed using Mathbaria pond water, a known reservoir of V. cholerae O1 (Alam et al., 2006a) and chitin chips.

^cVibrio cholerae O1 polyvalent and monoclonal antisera.

^dAttachment of V. cholerae O1 cells on chitin chip.

^eVibrio cholerae O1 cells from enrichment of HCl-treated homogenized chitin residues.

Mathbaria water, Mathbaria pond water microcosm, inoculated with V. cholerae O1 grown on Luria–Bertani broth and maintained at room temperature; MW–CC, Mathbaria pond water microcosm with chitin chips, inoculated with V. cholerae O1 grown on Luria–Bertani broth and maintained at room temperature; ND, not done; NG, no growth; BF, biofilms; CFU, colony forming unit; DFA, direct fluorescent antibody; TCBS, thiosulfate citrate bile sucrose agar; LB, Luria–Bertani agar.