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HPLC chromatograms of serum incubations of cetuximab-IRDye800CW. HPLC chromatograms at 280 nm (black line, A) and at 780 nm (blue lines, B-E) of cetuximab-IRDye800CW conjugates. Cetuximab-IRDye800CW (2.8 eq) incubated in PBS at 280 (A) and 780 nm (B). Cetuximab-IRDye800CW coupled with 1.5 (C), 2.8 (D), or 4.8 (E) eq of dye, incubated in serum for 96 h at 37°C days prior to HPLC analysis.
