Fig. 6.

Nitration of proteins in liver homogenates of APAP-treated WT (left) and KO (right) mice. Mice (n = 4) were treated with a 300 mg/kg dose of APAP and sacrificed at the indicated times, and livers were collected. The 0 time is the saline-treated control mice in both WT and KO mice. Hepatic homogenates were analyzed by Western blotting for the presence of nitrotyrosine (NT) protein adducts by using anti-3-nitrotyrosine antibody. GAPDH was used as a loading control. One representative gel is shown.