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. 2012 Jan;340(1):218–226. doi: 10.1124/jpet.111.187179

Fig. 2.

Fig. 2.

Ethanol (Etoh) inhibition of agonist-activated cysteine-substituted A7 mutants. A, traces show representative currents from single cells expressing A7C mutants [left, GluN1(A652C)/GluN2B; right, GluN1/GluN2B(A651C)]. Currents were activated by switching from normal recording solution to one containing glutamate and glycine alone (10 μM each; solid line, red and green traces) or with 100 mM ethanol (▩, black trace). B, dose-response curve for ethanol inhibition of agonist-evoked currents in cysteine-substituted A7 mutants. Data represent the mean ± S.E.M. from five to seven cells for each receptor combination. Estimated ethanol IC50 values were 101 mM GluN1(A652C)/GluN2A, 117.1 mM GluN1(A652C)/GluN2B, 102.9 mM GluN1/GluN2A(A650C), and 69.3 mM GluN1/GluN2B(A651C). See text for more details.