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Inhibition of nitric-oxide synthesis with l-NMA (100 μM) or stimulation of PKC with PMA (300 nM). Each increases AT1R expression in sympathetic nerve endings (cardiac synaptosomes) isolated from ex vivo guinea pig hearts subjected to I/R (20-min global ischemia + 4-min reperfusion). Top, representative Western blots. Bottom, quantification. Bars are means ± S.E.M. (n = 3–7). ***, P < 0.001 from I/R by ANOVA and Bonferroni's multiple test.
