Fig. 6.

H₃R activation attenuates the PMA-induced PKC activation (i.e., translocation from cytosol to membrane) in PC12-H₃ cells. Top, representative blots. Cells were treated with the PKC activator PMA (300 nM; 20 min), the H₃R agonist imetit (100 nM; 10 min), and the H₃R antagonist CBP (50 nM; 10 min). PKC expression in cytosolic (C) and membrane (M) fractions was determined by Western immunoblotting. Bottom, PKC translocation expressed as the ratio of PKC abundance between membrane and cytosolic fractions. Bars are means (± S.E.M.; n = 3) normalized relative to β-actin. *, P < 0.05 from control by ANOVA and Bonferroni's multiple test; ##, P < 0.01 from PMA by unpaired t test; †, P < 0.05 from PMA + imetit + CBP by ANOVA and Bonferroni.