Table 1.
Primer sequence and amplicon length of the genes studied with qRT-PCR
| UniGene | Gene name (symbol) | Left primer sequence | Right primer sequence | Amplicon length (nt) |
|---|---|---|---|---|
| Rn 92211 | RPL13a | GATGAACACCAACCCGTCTC | CACCATCCGCTTTTTCTTGT | 175 |
| Rn.1023 | Δ9-Desaturase (EC 1.14.19.1) | CTGGTATCCTTGGGTGTGGA | ACATAGGGGTGGAGGTAGGG | 100 |
| Rn.29724 | Elongase 1 (EC 2.3.1.119) | ATTCCTTCTCCCAGCCAGTT | GTGAGGAGAAAGGCCACAAG | 162 |
| Rn.46942 | Elongase 6 (EC 2.3.1.16) | GCTCTGCATTGGGTAAAC | TAATCTACGCAGGCCCTTTG | 162 |
The housekeeping gene RPL13a was used as reference gene for the relative expression calculations.