Fig. 4.

L1-ORF2 only is sufficient for retrotransposition in NHEJ-deficient cells. A schematic of the (A) pAJ101/L1.3 and (B) pJBM105 plasmid constructs. Hallmarks of the constructs are noted as in Fig. 1A except pJBM101/L1.3 constructs contain an mblastI reporter and a T7 tag on ORF1p (45). A PCR/Southern blot assay shows some clonal V3 cells harbor an ENi retrotransposition event at telomeres (A, pAJH230A/L1.3; and B, pJBM205/L1.3). A schematic of a representative PCR product positive for retrotransposition at telomeres from a clonal cell line is shown.