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. 2011 Dec 5;108(51):20597–20602. doi: 10.1073/pnas.1117184109

Fig. 3.

Fig. 3.

Maintaining the UPR resting state reveals severe defects in LHS1-, SCJ1-, and ALG5-deficient strains. (A) CPY and Gas1p biogenesis was analyzed in lhs1-1Δire1 and sec63-1 strains at 23 °C and 37 °C as described in Fig. 1A. (B) Pulse-chase analysis was performed at 37 °C to examine the degradation of CPY*-HA in WT, Δire1, scj1-1, and scj1-1Δire1 cells. The graph represents the mean ± SD of three independent experiments. (C) The biosynthesis of CPY was monitored in alg5-2Δire1 as described in Fig. 1C. rft1-2 cells were included to indicate positions of underglycosylated mCPY, which are denoted as “−1”, “−2”, “−3”, and “−4”, representing triply-, doubly-, singly-, and nonglycosylated species, respectively (11). “Mx” denotes the portion of the gel composed of p1, p2, and mCPY forms that are not easily differentiated. The other labels are described as in Fig. 1C. The immature form of CPY after Endo H digestion was expressed as a percentage of the total and is indicated.