Fig. 4.

The constitutive UPR activator HAC1ⁱ alleviates defects in the ts strains. (A) Cells with or without HAC1ⁱ-bearing plasmid were grown at 23 °C and serial dilutions of the culture were spotted onto duplicate selective SC plates. These plates were incubated at the indicated temperature until the appearance of colonies. (B) The synthesis of CPY and Gas1p was examined in lhs1-1Δire1 containing a HAC1ⁱ-bearing plasmid as described in Fig. 1A. (C) The degradation of CPY*-HA was analyzed in scj1-1, scj1-1Δire1, and scj1-1Δire1 with a HAC1ⁱ-containing plasmid at 37 °C. The graph was obtained from the mean ± SD of three independent experiments. (D) CPY biogenesis was monitored in Δalg5, alg5-2Δire1, alg5-2Δire1, and rft1-2 carrying HAC1ⁱ-bearing plasmid at 37 °C and labeled as described in Figs. 1C and 3C. The percentage of immature CPY compared to the total is shown below each section.