Fig. 6.

Complex of hTERT, BRG1, and NS/GNL3L is necessary for NS-induced TIC formation. (A) hTERT is required for NS/GNL3L-induced increase in CD44 expression. HeLa-NS cells or HeLa-GNL3L cells expressing either a control siRNA or two independent hTERT-specific siRNAs were immunoblotted for CD44. IB, immunoblot. (B) BRG1 is required for NS/GNL3L-induced increase in CD44 expression. CD44 expression was assessed by immunoblotting in HeLa-NS cells or HeLa-GNL3L cells stably expressing either a control vector or two independent BRG1-specific shRNAs. (C) hTERT is required for NS/GNL3L-induced increase in TWIST expression. HeLa-NS cells or HeLa-GNL3L cells expressing either a control siRNA or two independent hTERT-specific siRNAs were immunoblotted for TWIST. (D) BRG1 is required for NS/GNL3L-induced increase in TWIST expression. TWIST expression was assessed by immunoblotting in HeLa-NS cells or HeLa-GNL3L cells stably expressing either a control vector or two independent BRG1-specific shRNAs. (E) hTERT is required for tumor formation by HeLa cells. The number of tumors formed/number of injection sites in HeLa-NS cells or HeLa-GNL3L cells expressing either a control siRNA or two independent hTERT-specific siRNAs is shown. For E and F, 5 × 10⁴ cells were injected per mouse. NC, negative control. (F) BRG1 is required for tumor formation by HeLa cells expressing NS or GNL3L. HeLa-NS cells or HeLa-GNL3L cells expressing either a control vector or two independent BRG1-specific shRNAs are shown.