Table 2.

Kinetic parameters for Q160 mutants of S. shibatae IDI-2

Enzymes	kcat (× 103 s-1)	Km, μM	kcat/Km, M-1 s-1
Wild type	29.9 ± 3.5	7.39 ± 1.63	4050
Q160E	3.28 ± 0.31	8.26 ± 1.41	397
Q160H	1.21 ± 0.11	7.13 ± 1.22	170
Q160K	ND*	ND*	31.1†
Q160L	0.221 ± 0.014	1.52 ± 0.3	145
Q160N	0.197 ± 0.019	3.23 ± 0.75	61

^*ND, not determined.

^†Determined from the linear region of a v vs. [S] plot because of an apparently high .