Fig. 4.

Hbs1 preferentially targets recycling to ribosome complexes with short mRNAs. (A) Representative time courses of subunit splitting by Dom34∶Hbs1∶Rli1 on complexes containing mRNAs of increasing lengths. The length of mRNAs following the P-site codon is indicated. Subunit splitting was monitored by formation of peptidyl-tRNA using electrophoretic thin-layer chromatography. (B) Observed rate constants, as determined in A, for subunit splitting by the factors indicated within the sensitive window of mRNA lengths (n = 3, ± SEM). Length dependence is not observed in the absence of Hbs1.