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Rli1 accelerates eRF1∶eRF3 mediated recycling. (A) Representative time courses of the subunit-splitting activity of Rli1 and eRF1∶eRF3 on dipeptidyl-tRNA ribosome complexes containing 32P-labeled mRNA carrying a stop codon in the A site. Subunit splitting was monitored by native gel electrophoresis. Either eRF1(WT) or the eRF1(AGQ) variant was utilized. (B) Observed rate constants for subunit splitting mediated by eRF1(AGQ) in conjunction with other factors (n = 3, ± SEM).
