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. 2011 Nov 2;108(51):20784–20789. doi: 10.1073/pnas.1116795108

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Fig. 2. — Effects of alternative splicing on BK channel activation kinetics. (A) Average macroscopic currents evoked by 10-ms voltage steps from a −80 mV holding potential splice variants exposed to 5 μM Ca²⁺. Zero current is shown by the dashed line; light and dark traces are for A1- and A2-type variants, respectively. For clarity, only three traces are shown. Similar results were obtained for at least five patches per isoform. (B) Mean activation time constants for the splice variants shown in A. Open circles are A1-type variants, and filled circles are A2-type variants. Each point is the mean ± SEM (n ≥ 5). Differences between splice variant pairs were evaluated across the full voltage range by two-way ANOVAs and Bonferroni post hoc tests. Large asterisks () indicate significant main effect of the A exon variant by ANOVA (P < 0.001). *Significant differences (P < 0.01) between pairs of measurements at a single voltage by Bonferroni post hoc tests. ns, not significant (P > 0.01). F statistics and P values for ANOVAs were (Top to Bottom): F_(1,72) = 15.54, P < 0.001; F_(1,51) = 43.02, P < 0.001; F_(1,54) = 50.47, P < 0.001; F_(1,60) = 114.07, P < 0.001; F_(1,54) = 0.82, P = 0.37; and F_(1,66) = 25.07, P < 0.001.

Inline graphic — Effects of alternative splicing on BK channel activation kinetics. (A) Average macroscopic currents evoked by 10-ms voltage steps from a −80 mV holding potential splice variants exposed to 5 μM Ca²⁺. Zero current is shown by the dashed line; light and dark traces are for A1- and A2-type variants, respectively. For clarity, only three traces are shown. Similar results were obtained for at least five patches per isoform. (B) Mean activation time constants for the splice variants shown in A. Open circles are A1-type variants, and filled circles are A2-type variants. Each point is the mean ± SEM (n ≥ 5). Differences between splice variant pairs were evaluated across the full voltage range by two-way ANOVAs and Bonferroni post hoc tests. Large asterisks () indicate significant main effect of the A exon variant by ANOVA (P < 0.001). *Significant differences (P < 0.01) between pairs of measurements at a single voltage by Bonferroni post hoc tests. ns, not significant (P > 0.01). F statistics and P values for ANOVAs were (Top to Bottom): F_(1,72) = 15.54, P < 0.001; F_(1,51) = 43.02, P < 0.001; F_(1,54) = 50.47, P < 0.001; F_(1,60) = 114.07, P < 0.001; F_(1,54) = 0.82, P = 0.37; and F_(1,66) = 25.07, P < 0.001.