Skip to main content
. 2011 Dec 5;108(51):20713–20718. doi: 10.1073/pnas.1118526109

Fig. 1.

Fig. 1.

Isx induces expression of insulin and transcription factors in primary cultured human islets. (A) Primary human islets cultured in RPMI 160 for 6 mo were treated with vehicle or 40 μM Isx for 1 or 2 d. Expression of human insulin and glucagon mRNA was assessed. (B) Insulin secretion measured by ELISA over 24 h from islets treated with Isx or vehicle for 1 or 2 d. (C) Total insulin content of islets cultured for 3 mo and fresh islets, each treated for 48 h with Isx or DMSO. (D) Time course of pancreatic gene induction in islets cultured for 1 y and treated with DMSO for 8 d or with 40 μM Isx for 2, 4, or 8 d. (E) Immunohistochemical staining of insulin, NeuroD1, Ngn3, and MafA of islets cultured for 2 mo and treated with Isx or vehicle for 48 h. Nuclei were stained with DAPI. Vehicle- and Isx-treated samples were compared using Student's t test (*P < 0.05; **P < 0.01).