Fig. 3.

The evolved isolates Re04 and Re05 possessed more rigid and impermeable cell walls than the parent strain (Mr2575-GFP), and were better adapted to field conditions. To determine susceptibility of Mr2575-GFP, Re04, and Re05 to Congo red, 5 μL of each spore suspension (range 5 × 10 conidia/mL to 5 × 10⁴ conidia/mL) was spotted onto PDA (A) or PDA complemented with Congo red (300 μg/mL) (B). The sensitivity to Congo Red (CR) is determined by comparing the extent of colony formation. To measure population levels, Re04, Re05 and the introduced strain Mr2575-GFP were collected from turf plots and CFUs were counted (C). Error bars indicate SD, n = 15.