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Both endogenous BBS3 and the BBSome associate with membranes. Shown are Western blots of sucrose gradient fractions from mechanically disrupted testicular tissue. Both endogenous Bbs3 and the BBSome are associated with membranes (A). Loss of Bbs3 does not affect the general membrane association of the BBSome (B). Compared with WT (C), loss of Bbs4 does not affect the general membrane association of Bbs3 (D).
