Fig. 6.

BBS3 is required for proper Smo ciliary localization and modulates Shh pathway activation. Cultured MEF cells from WT (A) and Bbs3^−/− (B) mice were stained with antiacetylated tubulin (green) as a marker for cilia and anti-Smo (red). (C) Quantitation of percentage of cilia with Smo staining when treated with DMSO or SAG. (D) Quantitative PCR for Gli1 from cultured Bbs3^−/− MEF cells stimulated with SAG showed a 22.3% decrease compared with WT MEF cells when normalized to actin. *P < 0.03. (Scale bars, 10 μm.)