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. Author manuscript; available in PMC: 2012 Jan 4.
Published in final edited form as: Biochemistry. 2010 May 4;49(17):3743–3752. doi: 10.1021/bi100135d

Figure 2. Biological activity of heparin and HS bound fractions.

Figure 2

The ATIII mediated inhibitory activity of heparin and HS octasaccharides on FXa was studied using an in vitro colorimetric assay. FXa is able to hydrolyze a synthetic substrate yielding a colored product detected at 405nm. When FXa is incubated with its substrate in the presence of ATIII and biologically active heparinoids, its hydrolytic activity is inhibited. The 405nm absorbance is inversely related to the anti-FXa activity of heparinoid octasaccharides used in the experiments. Experiments were done in triplicate.