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Primer sets used to generate full length of 3′UTRs of Hsp70 family genes or to measure Grp78 mRNA level
| Forward Primer (5′ to 3′) | Reverse Primer (5′ to 3′) | ||
|---|---|---|---|
| 3′UTR | HSPA1A | TAGAGGCCTCTGCTGGCTCT | TGGCAAGTGATTTTGAAGTTTATTTTC |
| HSPA5 | GTGCACTGATCTGCTAGAGC | CTTAAATTTTAGTTATCTAAAATAAAAGATGG | |
| HSPA9 | TAATAGCAGAAATTTTGAAGCCAGAAG | GGAACCCAGATTTCTTTCCCTA | |
| mRNA | grp78 | GGAAAGAAGGTTACCCATGC | GGAACAGGTCCATGTTCAGC |
| actin | GGCTGTGTTGTCCCTGTAT | CCGCTCATTGCCGATAGTG |
