Dihydroethidium (DHE) staining of kidney frozen sections of rats (n = 5) subjected to normal salt (NS) intake (A), chronic ANG II infusion (B), high salt (HS) intake (C), ANG II chronic infusion associated with a HS diet (ANG II+HS; D), and chronic ANG II infusion plus HS diet plus treatment with ANG II type 1 receptor blocker (ANG II+HS+ARB; E). DHE nuclear staining denotes the presence of reactive oxygen species (ROS). DHE fluorescence, quantified as described in methods, in kidney sections using ×200 magnification, is represented in F. Values are means ± SE. *P < 0.05 vs. NS.