Fig. 2.

Effects of HS diet in chronic ANG II-infused rats on NAPDH oxidase activity (A) and peroxynitrite activity (B) in renal tissues. Rat groups (n = 5) were divided as described in methods. NAPDH oxidase activity was quantified as relative luminescent unit (RLU) per minute. Nitrotyrosine residues were detected by Western blot analysis using the an anti-nTyr antibody (1:1,000; Cayman Chemical, Ann Arbor, MI), and GAPDH was used as a loading control. Quantification of the total protein bands ranging from 120 to 15 kDa was obtained by the Image J Software (NIH) and expressed as a ratio from GAPDH. Values are means ± SE. *P < 0.05 vs. NS.