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Kidney immunoexpression of CD68, a marker of macrophages, in glomeruli (×400) and tubulointerstitial areas (×200; A). Twenty different microscopic fields per tissue section per animal (n = 3/group) were analyzed from paraffin kidney sections (3 μm). The number of CD68-positive cells/mm2 was quantified by an automatic image analysis in glomeruli (B) and tubulointerstitium (C) and expressed as fold-changes relative to NS. Values are means ± SE. *P < 0.0001 vs. NS. **P < 0.0001 vs. ANG II.
