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Wtip knockdown affects dynamic actin assembly. Time-lapse images of shEMP (A) and shWtip cells (B) transiently transfected with eGFP-actin (green) are shown. Arrows indicate stress fiber formation in shEMP cells, whereas arrowheads indicate actin clusters and the lack of actin stress fiber formation in shWtip cells. C: quantification of stress fiber formation in shEMP vs. shWtip cells (*P < 0.05). Scale bars = 20 μm.
