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. 2012 Jan 4;7(1):e28232. doi: 10.1371/journal.pone.0028232

Figure 3. B7-H4 inhibits phosphorylation of AKT on different T cell subsets similarly.

Figure 3

Naïve CD3+, CD4+, or CD8+ T-cell subsets were stimulated with 0.3 µg/ml of plate-bound anti-CD3 and soluble anti-CD28 (1 µg/ml) for 16 h and then rested for 30 h prior to re-activation. Activated CD3+, CD4+, or CD8+ T-cell subsets were stimulated with plate-bound anti-CD3 (5 µg/ml) and soluble anti-CD28 (2 µg/ml) for 10 min. Representative western blot of protein extracts from CD3+, CD4+, or CD8+ T-cell subsets were detected by phosphorylated AKT Ser473 (A) and quantitated by using Bio-Rad Quantity One program. The y-axis was normalized for the loading control. B7-H4 treatment significantly inhibited AKT phosphorylation at 10 min to a similar degree on different T-cell subsets (B). Data represent 3 independent experiments and are expressed as means ± SD from 3–4 mice per group.