Table 1. Iron-responsive Fur regulon in M. gryphiswaldense.
Gene | Function | RT-PCR | Product | |
DP | fur | |||
EF120624.1 | Iron acquisition system: Fe2+ transporter (Feo) | 1.08 | 7.66 | Ferrous iron transport protein(FeoAB1) |
MGR_1447-1446 | Iron acquisition system: Fe2+ transporter (Feo) | −1.32 | 4.55 | Ferrous iron transport protein(FeoAB2) |
MGR_3446 | Catalyze dismutation of superoxide anions | −1.48 | 2.09 | Superoxide dismutase |
MGR_4274 | Catalase-peroxidase | 1.94 | 1.76 | Catalase-peroxidase |
DP, the comparison of mRNA from WT cells treated with 30 µM iron chelator 2, 2′-dipyridyl (DIPy) and cells added with 60 µM ferric citrate.
fur, the comparison of mRNA expression in the fur mutant (F4) and WT grown under high-iron condition.
Positive and negative numbers indicate fold increase or decrease, respectively.