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. 2012 Jan 4;7(1):e29371. doi: 10.1371/journal.pone.0029371

Figure 1. BAFF-R deficiency attenuates selectively conventional B2 cells, not peritoneal B1a cells.

Figure 1

(A) PCR analysis of BAFF-R gene disruption was performed using DNA extracted from tails of BAFF-R−/− ApoE−/−, BAFF-R+/+ ApoE−/− and BAFF-R+/+ ApoE+/+ mice. (B) BaffR.ApoE DKO mice showed that CD22+ B cells were lowered in peripheral blood, peritoneal cavity, spleen and peripheral lymph nodes. (C) FACS analysis on peritoneal cavity revealed that depleted B cells were conventional CD22+ CD5- B2 cells, not CD22+ CD5+ B1a cells comparing to ApoE KO mice. (D) A representative FACS analysis of peritoneal cavity showed that only conventional CD22+ CD5- B2 cell population (left upper quadrant) was decreased in BaffR.ApoE DKO and peritoneal CD22+ CD5+ B1a cell (right upper quadrant) was unaffected. Open bar = ApoE KO; Black bar = BaffR.ApoE DKO; n = 9–11 mice; **: p<0.01, ***: p<0.001.