Figure 6. Kinase phosphorylation induced by engagement of CD16 or co-engagement of 2B4 and DNAM-1.

CD16- (two proteome experiments) or 2B4 and DNAM-1 co-activated (three proteome experiments) NK cells from two healthy human donors were analyzed in this study. (A) Kinases, which have previously been associated with NK cell signaling and/or function. (B) Putative novel CD16- and/or 2B4 and DNAM-1-dependent signaling components. ¹⁾UniProt name of kinases and phosphorylation site annotation; regulation of phosphorylation site following ²⁾CD16 engagement or ³⁾2B4 and DNAM-1 co-activation is indicated as follows: green, up-regulated; red, down-regulated; gray, no statistical significant phosphorylation site regulation was detected; Number of (filled) circles indicates how often the significant regulation of a respective phosphorylation site was detected according to iTRAQassist (no overlap of protein and respective phosphopeptide regulation curves). Phosphorylation sites regulated with the same tendency, but not matching the criteria of statistical significance are marked as open circles. Proteome experiments that failed to detect a respective phosphorylation site are indicated by n.d. (not detected).