Table 2. Parameters impacting throughput.
| Scan pattern | single | 2×2 | 3×3 | 4×4 | 5×5 |
| Z'-factor Averages | 0.67 | 0.77 | 0.79 | 0.76 | 0.78 |
| Scan Time (96 well per scan) | 00∶26 | 1∶34 | 2∶49 | 3∶40 | 8∶37 |
| Scan Time per subject (96 well) | 0.27 sec | 0.94 sec | 1.76 sec | 2.29 sec | 5.39 sec |
| Estimated daily capacity per reader* | >200,000 | >75,000 | >45,000 | >35,000 | >15,000 |
| Number of Flashes ** | 2 | 5 | 10 | 20 | 50 |
| Z'-factor Averages | 0.71 | 0.85 | 0.86 | 0.87 | 0.87 |
| Scan Time (96 well per scan) | 00∶23 | 0∶26 | 0∶31 | 0∶41 | 1∶13 |
| Scan Time per subject (96 well) | 0.24 sec | 0.27 sec | 0.32 sec | 0.43 sec | 0.76 sec |
| Estimated daily capacity per reader | >215,000 | >200,000 | >180,000 | >140,000 | >90,000 |
Constants: Settle time = 40 ms, Limited to green, yellow and red fluorophore expressing lines.
*Calculated by adding 15 seconds robotics handling time per 96 well plate.
**Single scan pattern used for all calculations.