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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Cancer Res. 2011 Nov 11;72(1):294–303. doi: 10.1158/0008-5472.CAN-11-3240

Fig. 4. Noxa induction by Pim inhibitors is mediated through the endoplasmic reticulum stress response.

Fig. 4

A, LNCaP cells were treated with DMSO, 10 µM SMI-4a, or 10 µM K00135 for 16h. Cell extracts were analyzed by immunblotting with antibodies shown. B, LNCaP cells were treated DMSO or SMI-4a for 16h. Aliquots of cell extracts were subjected to immunoprecipitation (IP) with Mcl-1 antibody and then immunoblot (IB) probed with Mcl-1 and Noxa antibodies. C, LNCaP cells were treated with 10 µM SMI-4a for 0, 4 or 16h. Cell extracts were analyzed by immunblotting with antibodies shown. D, LNCaP cells were treated with various doses of SMI-4a (4a) for 16h. Cell extracts were analyzed by immunblotting with antibodies shown. E, LNCaP cells were treated with Pim inhibitors at the indicated doses for 16h. Tunicamycin (TM) and Thapsigargin (Tg) were used as controls. Cells were treated with 10058-F4, which is of the same chemotype as SMI-4a and is an active Pim inhibitor. Cell extracts were analyzed by immunblotting with antibodies shown.