Table 2.

Surface phenotype of CD8⁺ T-cell clones

%Positive	Day 8*			Day 15*			Day 22*
	Treg	Non Treg	p-Value	Treg	Non Treg	p-Value	Treg	Non Treg	p-Value
CD11a	100±0	100±0	n.a.	100±0	100±0	n.a.	100±0	100±0	n.a.
CD18	n.a.	n.a.	n.a.	n.a.	n.a.	n.a.	100±0	100±0	n.a.
CD69	41±16	57±37	0.45	61±19	81±16	0.16	69±13	81±17	0.33
CD16	0.7±0.2	2.0±2.4	0.33	0.2±0.1	1.1±1.2	0.17	0.5±0.1	1.2±0.7	0.10
CD161	13±23	7.6±12	0.71	15±28	8.2±13	0.67	18±33	12±17	0.74
CD56	45±28	25±16	0.25	50±30	34±25	0.43	56±28	30±23	0.19
(CD56+ MFI)**	(27±13)	(13±2)	(0.06)	(16±7)	(8±3)	(0.09)	(17±7)	(7±2)	(<0.05)
CD62L	45±25	50±33	0.83	6.7±11	7.5±9.0	0.91	3.7±5.1	4.5±4.5	0.82
CD95	49±19	61±22	0.43	14±4.8	30±24	0.25	10±6.2	23±16	0.19
CD27	0.5±0.1	2.2±3.0	0.30	1.1±0.4	1.1±0.8	0.96	1.5±0.9	1.3±1.0	0.86
CD28	0.4±0.2	4.3±5.3	0.19	0.2±0.1	0.7±0.8	0.24	1.0±0.3	2.2±2.1	0.31
CD122	4.7±3.6	3.5±2.7	0.63	0.6±0.2	1.4±1.1	0.23	1.9±0.3	3.2±1.9	0.25
CXCR4	1.0±0.6	4.7±6.3	0.27	0.1±0.1	0.3±0.2	0.14	0.6±0.4	1.1±0.9	0.35
CCR7	5.7±1.6	12±9.1	0.24	3.1±1.4	4.3±2.1	0.39	5.2±1.0	3.8±1.8	0.22

CD8⁺ Treg-cell clones: 1B3, 1E2, 1E8, and 1F3; non-Treg-cell clones: 1B1, 1B6, 1F1, and 1D1.

^*, CD8⁺ T-cell clones were analyzed 8, 15 and 22 days after PHA stimulation in the presence of feeder cells:

^**MFI of CD56 positive subsets; n.a., not available. The results are shown as percentage of positive cells (mean±SD).