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Five ng/ml TGF-β1 promoted high expressions of lysyl oxidase-like (LOX) proteins in both anterior cruciate ligament (ACL) and medial collateral ligament (MCL), and elevated mRNA peaks appeared within 24 h after TGF-β1 treatments by real-time quantitative PCR (mean ± SD) (n = 3). An mRNA peak appeared at 12 h in LOX and LOXL-3; for LOXL-1, LOXL-2, and LOXL-4 the peak appeared at 6 h. *Significant difference with respect to control (p < 0.05)
