Table 1.

Primer sequences for RT-PCR

Target genes	Annealing temperature (°C)	Primer sequences	Product size
Runx2	62	Forward: GAC AGA AGC TTG ATG ACT CTA AAC C	171
		Reverse: TCT GTA ATC TGA CTC TGT CCT TGT
Collagen Type I	60	Forward: GCA TGG CCA AGA AGA CAT CC	83
		Reverse: CCT CGG GTT TCC ACG TCT C
Osteocalcin	60	Forward: ATG AGG ACC CTC TCT CTG CTC	270
		Reverse: ATG AGG ACC CTC TCT CTG CTC
GAPDH	52	Forward: AAC TAC ATG GCT ACA TGT TCC A	73
		Reverse: CCA TTC TCG GCC TTG ACT GT