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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Clin Cancer Res. 2011 Nov 16;18(1):290–300. doi: 10.1158/1078-0432.CCR-11-2260

Figure 4. Radiation-induced senescence is associated with p21 expression and dependent on prolonged ROS production.

Figure 4

(A) p21 protein expression and p21 luciferase reporter activity in UMSCC1 cells expressing the indicated TP53 constructs treated with the indicated doses of radiation for 24h unless otherwise stated. (B) ROS production measured as indicated in the methods after 2 Gy of radiation. (C) Percentage of cells either SA-Gal positive (S alone), exhibiting micronuclei (MC alone) or both at 4 days after 4 Gy of radiation. Cells were treated with the indicated dose of NAC starting 2 h after radiation and treatment continued overnight. (D) Clonogenic survival after 2 Gy of radiation. Cells were treated with NAC in an identical manner to (C). * - significantly increased over unirradiated control (p<0.05), + - significantly different from UMSCC1 wild type in the same group (p<0.05). # - significantly increased compared to non-NAC treated group (p<0.001).

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