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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Alcohol Clin Exp Res. 2011 Sep 26;36(3):432–442. doi: 10.1111/j.1530-0277.2011.01640.x

Figure 6.

Figure 6

Immunofluorescence of ZO-1 (red), claudin-1, claudin-5 and claudin-7(green) in NHBE, exposed to 100mM alcohol for 24 hours ± Gö-6976, a PKCα inhibitor, or myristolated-Zeta, a PKCζ inhibitor (Note: Images are compressed Z stacks). Both ZO-1 and claudin-1 are localized to the cell membrane in control cells. Exposure to 100mM alcohol reduced ZO-1, claudin-1, claudin-5 and claudin-7 localization at the plasma membrane in alcohol-treated NHBE cells. In addition there also appears to be a shift in membrane localization of ZO-1 and claudins to a cytoplasmic localization in NHBE cells. Pretreatment with Gö-6976 prevented ZO-1, claudin-1, claudin-5, and claudin-7 alcohol-induced protein changes, while myristolated- Zeta did not. Bar equals 10μm.