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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Cancer Res. 2011 Nov 17;72(1):346–355. doi: 10.1158/0008-5472.CAN-11-2819

Figure 7. Notch and Hedgehog pathways were upregulated in DTs.

Figure 7

Fluorescent immunohistochemistry was performed on paraffin-embedded DTs and showed positive expression of the Notch target gene Hes-1 (green) (A) and Notch-1 (red) (B). Co-localization with BMI-1 (red) produced yellow nuclei (A). DT-derived cells also expressed the transcriptional repressor BMI-1 (C). Co-localization of red staining of BMI-1 with the blue counter-stain produced purple-colored nuclei. Similarly, purple nuclei were evident when the MSCs were stained with antibodies for the Hedgehog transcriptional activator, Gli-1 (red) (D).