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. 2011 Dec 1;106(1):92–98. doi: 10.1038/bjc.2011.538

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Copyright © 2012 Cancer Research UK

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Conjugation of lysates to VLP enhances delivery of tumour antigens to DC antigen processing compartments. (A) Subcellular fractions containing early endosomes (EE), late endosomes (LE), lysosomes (Lys) or recycling endosomes (RE) were prepared from DC. The following probes were used as markers of specific subcellular compartments; EEA1 (EE), Rab7 (LE), LAMP1 (Lys) and Rab11 (RE). (B) Dendritic cells were incubated with VLP for 1 or 3 h. After washing, subcellular fractions were prepared and the presence of VLP was analysed by western blot using anti-VP60 antibodies. (C) Dendritic cells were incubated with lysate for 1 or 3 h. After washing, subcellular fractions were prepared and the presence of MART1 was analysed by western blot. (D) Dendritic cells were incubated with VLP-lysate for 1 or 3 h. After washing, subcellular fractions were prepared and the presence of MART1 was analysed by western blot. Results are representative of four independent experiments.