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Isothermal titration calorimetry of the wild-type DDAH-1•4 complex. Top panel shows raw data output for titration of 4 into DDAH-1 at 25° C. Bottom panel shows integrated values from the top panel, as best fit to a single-site binding model to give: Ka = 1.4 ± 0.2 × 105 M−1, ΔH° = − 5580 ± 180 cal/mol, ΔS° = 4.79 cal/mol/deg, n = 0.6. See experimental section for details.
