Table 3.
Genotypic characterization of 31 MRSA isolates collected during summer 2010.
| Date | Isolate | Sample Type | Sitea | SCCmec Typeb | MLSTc | PFGE Type | Genotype | |
|---|---|---|---|---|---|---|---|---|
| Beach A [n = 20 strains] | 24Jun | 111 | Sand | 11 | IV | 8 | K | erm(C), msr(A), aadD |
| 118 | Fresh | NA | IV | 15 | tet(M), tet(K), erm(C), msr(A), aadD | |||
| 125 | Fresh | NA | IV | 133 | C | tet(K), erm(C), msr(A), aadD | ||
| 07Jul | 302 | Marine | NA | IV | 109 | F | tet(M), tet(K), erm(A), msr(A), aadD | |
| 308 | Marine | 2 | IV | 109 | F | tet(M), erm(A), erm(C), msr(A), aadD | ||
| 361 | Sand | 11 | IV | 109 | F | tet(M), erm(A), erm(C), msr(A), aadD | ||
| 08Jul | 401 | Fresh | NA | IV | 5 | L | tet(M), tet(K), erm(C), msr(A), aadD | |
| Seep1 | Sand | NA | IV | 88 | tet(M), tet(K), msr(A), aadD | |||
| 3mid | Sand | NA | IV | 15 | G | tet(M), tet(K), msr(A), aadD | ||
| 12Jul | 512 | Fresh | 27 | IV | 133 | E | tet(K), erm(C), msr(A), aadD | |
| 515 | Fresh | 28 | IV | 1875 | tet(K), erm(C), msr(A), aadD | |||
| 526 | Fresh | 30 | IV | 1875 | tet(M), tet(K), msr(A), aadD | |||
| 19Jul | 813 | Fresh | 17 | IV | 1875 | tet(M), tet(K), erm(C), msr(A), aadD | ||
| 823 | Fresh | 27 | NT | 1875 | tet(M), tet(K), erm(C), msr(A), aadD | |||
| 824 | Fresh | 28 | NT | 133 | tet(K), erm(C), msr(A), aadD | |||
| 827 | Fresh | 30 | IV | 1956 | I | tet(K), erm(C), msr(A), aadD | ||
| 02Aug | 1112 (1) | Fresh | 16 | I | 133 | B | none | |
| 1112 (2) | Fresh | 16 | II | 45 | M | erm(A), aadD | ||
| 1113 | Fresh | 17 | I | 1956 | J | none | ||
| 1124 | Fresh | 28 | I | 1956 | aadD | |||
|
| ||||||||
| Beach B [n =5 | 02Jul | 252 | Fresh | 19 | IV | 133 | B | tet(K), erm(C), msr(A), aadD |
| 11Jul | PC3 | Fresh | NA | NT | 6 | tet(M), tet(K), msr(A), aadD | ||
| 22Jul | 1012 | Fresh | 12 | NT | 6 | tet(M), tet(K), msr(A), aadD | ||
| 1017 | Fresh | 17 | NT | 2049 | erm(C), aadD | |||
| 1019 | Fresh | 19 | NT | 2049 | tet(K), erm(C), msr(A), aadD | |||
|
| ||||||||
| Beach C [n = 6 strains] | 01Jul | 244 | Fresh | 1 | IV | 133 | A | erm(C), msr(A), aadD |
| 248 | Fresh | 4 | IV | 133 | A | tet(K), msr(A), aadD | ||
| 257 | Sand | 7 | IV | 97 | N | tet(K), erm(C), msr(A), aadD | ||
| 14Jul | 603 | Sand | 7 | IV | 30 | H | tet(M), tet(K), erm(C), msr(A), aadD | |
| 21Jul | 909 | Fresh | 5 | IV | 133 | D | tet(K), erm(C), msr(A), aadD | |
| 910 | Sand | 9 | IV | 133 | D | tet(K), erm(C), msr(A), aadD | ||
Isolates with the same SCCmec type, MLS and PFGE type were considered to be genetically related and based on these the 31 isolates represent 21 different strains
a
Site number corresponds with figure 1; NA: not applicable
b
SCCmec type: I – V, NT [non-typable]. No SCCmec type IV related to USA300 [homology < 50%]
c
MLST type determined by the allelic profile of seven housekeeping gene arc, aro, glp, gmk, pta, tpi, and ygi.
ST5, ST6, ST8, ST15, ST30, ST45, ST88, ST97, ST109 have all been found in humans and animals; ST133 has been found in livestock; ST1956 has been found in squirrels; ST1875 and ST2049 are new MLST types isolated in the study.