Location of the phyA-5 mutation. Domains of phytochromes are shown (not to scale) together with the sequence alignment of the corresponding region. The position of the phyA-5 missense mutation is indicated (boldface letter). For database accession numbers, see “Materials and Methods.” PASN, N-terminal PER/ARNT/SIM domain; GAF, cGMP-specific phosphodiesterases, adenylyl cyclases, and FhlA domain; PHY, phytochrome domain; PAS1 and PAS2, two additional PER/ARNT/SIM domains; HKRD, His kinase-related domain. The small black rectangle attached to GAF represents the chromophore.