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. 2011 Oct 3;158(1):107–118. doi: 10.1104/pp.111.186288

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© 2012 American Society of Plant Biologists. All rights reserved.

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Figure 7. — Expression of PHYA-5-YFP-NLS or PHYA-YFP-NLS equally complements the phyA-201 mutant. A, Transgenic Arabidopsis seedlings expressing the indicated chimeric proteins under the control of the PHYA promoter in the phyA-201 background were grown for 4 d in darkness prior to epifluorescence microscopy. Arrows point to nuclei. Bar = 10 μm. B, Hypocotyl lengths of seedlings grown under 1 μmol m⁻² s⁻¹ constant FR light for 4 d were measured. Analyzed genotypes are as follows: bar 1, Ws; bar 2, phyA-5; bar 3, phyA-201; bar 4, PHYA:PHYA-YFP in phyA-201; bar 5, PHYA:PHYA-5-YFP in phyA-201; bar 6, PHYA:PHYA-YFP-NLS in phyA-201; bar 7, PHYA:PHYA-5-YFP-NLS in phyA-201. Error bars indicate se. C, Seedlings grown for 4 d in the dark (lanes 1 and 3) or under 1 μmol m⁻² s⁻¹ FR light (lanes 2 and 4) were subjected to total protein extraction and western-blot hybridization applying PHYA (top panel) or ACTIN-specific antiserum (bottom panel). The analyzed genotypes are PHYA:PHYA-YFP-NLS in phyA-201 (lanes 1 and 2) and PHYA:PHYA-5-YFP-NLS in phyA-201 (lanes 3 and 4).