Figure 5.

Molecular characterization of transgenic YellowA fruit overexpressing the ACT1A-1 cDNA from Hayward. A, Proteins were extracted from mature fruit and separated by 1D SDS-PAGE (2 μg per lane). Molecular mass standards (in kD) were SeeBluePlus2 (Invitrogen). B, Western analysis of actinidin levels in wild-type, control, and transgenic fruit. Actinidin was detected using an actinidin-specific polyclonal antibody. C, CP activities were measured using the fluorescence assay of Rassam and Laing (2004). Data were normalized to 0.1 mg mL⁻¹ protein per assay and are shown with the activity of Hayward set at 100%. Samples are as follows: Hay, Hayward wild type; YA, YellowA wild type; T1 to T4, transgenic YellowA lines overexpressing the ACT1A-1 cDNA from Hayward; C1 and C2, control YellowA lines.