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. 2011 Nov 22;158(1):95–106. doi: 10.1104/pp.111.187062

Table I. Some useful multicolored FP probes for visualizing the nucleus and subnuclear components in living plant cells.

C, Cyan; G, green; Y, yellow; R, red; X, many different colors.

Target FP Color Fusion with Reference
Nucleus G NLS-sGFP::GUS Grebenok et al. (1997)
Minimal NLS from c2 polypeptide of tobacco, fusion to GUS prevents size-related diffusion from the nucleus Chytilova et al. (1999)
Y Histone H2B
C-terminal fusion to the full-length cDNA of the Arabidopsis H2B gene Boisnard-Lorig et al. (2001)
G/R PopP2
N-terminal fusion to the full-length genomic PopP2 gene of Ralstonia solanacearum Deslandes et al. (2003)
G/Y HTA6 (histone H2A)
C-terminal fusion to the full-length cDNA of the Arabidopsis HTA6 gene Zhang et al. (2005)
Y/C pAtHB8-NLS-YFP and pAtHB8-CFP-NLS promoter region of AtHB8 (1,997 bp upstream of ORF) and SV40 NLS Sawchuk et al. (2007)
G LeMAF1-GFP-LeMAF1
Full-length cDNA of the Lycopersicon esculentum (cv VFNT cherry) MFP1 associated factor1 (MAF1) gene Gindullis et al. (1999)
G SM40::GFP
C-terminal fusion of Mammalian simian virus 40 large T-antigen nuclearlocalization signal (amino acid residues 126–132) Chiu et al. (1996)
Nuclear envelope G AtRAN-GAP1
C-terminal fusion to the full-length cDNA of Arabidopsis RanGTPase-activating protein1 (RAN-GAP1) Rose and Meier (2001)
G RAN-GAP1 and RAN-GAP2
C-terminal fusion to the full-length cDNA of Arabidopsis RanGTPase-activating protein1(RAN-GAP1) and RAN-GAP2 Pay et al. (2002)
G Lamin B receptor
C-terminal fusion to the first 238 amino acids of the Homo sapiens Lamin B receptor Irons et al. (2003)
G AtSad1a and AtSad1b
C-terminal fusion to the full-length cDNA of Arabidopsis Sad1a and b genes Van Damme et al. (2004)
G MOS3
C-terminal fusion to the full-length cDNA of Arabidopsis MOS3 (MOFIDIER OF SNC1) Zhang and Li (2005)
G AtSUN1 (Sad1/UNC84) Graumann et al. (2010)
AtSUN2 Graumann and Evans (2011)
Nuclear pores G Nup136/Nup1
C-terminal fusion to the genomic and full-length cDNA of Arabidopsis Nup136 Tamura et al. (2010); Tamura and Hara-Nishimura (2011)
Nucleolus G AtFbr1::smGFP
C-terminal fusion to the full-length cDNA of Arabidopsis FIBRILLARIN1 Pih et al. (2000)
G Nop10 (At2g20490) Lorković et al. (2004)
Cajal bodies G U2B” (At2g30260) Boudonck et al. (1999)
G ORF3 Kim et al. (2007)
AtCoilin Kim et al. (2007)
Nuclear bodies/speckles G/R Arabidopsis CRYPTOCHROME 2 (CRY2-At1g04400) Yu et al. (2009)
X Different SR proteins Lorković et al. (2004)
G COP1 (At2g32950) Wang et al. (2001); Yang et al. (2001)
Chromatin G LIKE HETEROCHROMATIN PROTEIN1 (LHP1: At5g17690) Libault et al. (2005); Zemach et al. (2006)
Heterochromatin G Heterochromatin protein 1 (HP1γ human) Fass et al. (2002)
Euchromatin G Arabidopsis histone deacetylase 1 (AF014824) Fong et al. (2006)
Centromere G Polydactyl zinc finger (PZF) DNA binding domains Lindhout et al. (2007)
G Histone3 (HTR12; At1g01370) Fang and Spector (2005)
C Histone 2B (HTB1:At1g07790) Fang and Spector (2005)
Y Histone H3 (CENH3) Moraes et al. (2011); Ingouff et al. (2007, 2010)