Figure 2.

Multiple gene expression in protoplasts from triple- and quadruple-gene long binary vectors. EYFP-CHS expression was targeted to the rough endoplasmic reticulum (shown in yellow in A and E), while ChrD-RFP was targeted to the chloroplasts (shown in red in B, F, I, and K) and overlapped with the chloroplast autofluorescence (shown in blue in C, G, J, and L). DsRed2-P was targeted to the cytoplasm (shown in red in B and I) and was redirected into the nucleus in the presence of free N protein to form subnuclear aggregates (shown in red in F and K). D and H present merged signals of EYFP, DsRed2, RFP, and plastid autofluorescence. I and J are magnifications of B and C; K and L are magnifications of F and G. All panels are single confocal sections.