Skip to main content
. 2011 Nov 11;158(1):252–263. doi: 10.1104/pp.111.186544

Figure 6.

Figure 6.

SN1 self-interacted in vivo by BIFC. A, Schematic representation of the cassette vectors used for agroinfiltration assays: SN1-YFPN, SN1-YFPC, CP-YFPN, CP-YFPC, E-YFPN, and E-YFPC. SN1, SN-1 open reading frame; 35S, cauliflower mosaic virus-35S promoter; Nos-T, nopaline synthase terminator; CP, tobacco mosaic virus coat protein (CP)T42W (a variant of CP); E, Empty; YPFN, fragment of YPF encoding amino acids 1 to 155; YPFC, fragment of YPF encoding amino acids 155 to 239. Diagrams are not to scale. B, Scheme of the combination of constructs coexpressed in BiFC assays. C to F, YFP epifluorescence microscopy images of N. benthamiana epidermal cells of agroinfiltrated leaves with a mixture of Agrobacterium strains harboring constructs encoding fusion proteins. Each image is representative of several experiments: negative controls (C), positive control (D), SN1 tested interaction (E), and epidermal cells under white light (F). Bar = 10 μm.