Figure 6.

Protein S-nitrosylation is involved in H₂O₂-induced leaf cell death. A to D, OsGSNOR relative expression level (with the noe1 value set as 1.0; A), GSNOR activity (B), SNO content (C), and S-nitrosylated proteins from biotin-switch assay immunoblotted with anti-biotin antibody (D) using 4-month-old wild-type (WT), noe1, and OsGSNOR noe1 transgenic plants grown under high light (1,600 μmol m⁻² s⁻¹). E, Symptoms of 10-d-old wild-type, noe1, and OsGSNOR noe1 transgenic plants after treatment with 200 μm PTIO, 2.5 mm SNP, and H₂O₂ (generated by 2.5 mm Glc plus 2.5 units mL⁻¹ Glc oxidase) for 3 d under low light (400 μmol m⁻² s⁻¹). These data were obtained from three independent replicates. * P < 0.05, ** P < 0.01 (t test). DTT, Dithiothreitol.